Generator

Part:BBa_K902048

Designed by: Emily Hicks, Stephanie Willatt   Group: iGEM12_Calgary   (2012-10-02)

Constitutive XylE generator

XylE codes for the extra-diol dioxygenase called catechol 2,3-dioxygenase1. This enzyme converts catechol to 2-hydroxymuconic semialdehyde, by cleaving catechol adjacent to the two hydroxyl groups1.Because catechol is colourless and 2-HMS is a yellow compound visual assays can be used to monitor the activity of XylE. The tetR promoter (R0040) was used instead of the glucose repressible cstA promoter (K118011) so that the transformed cells could be grown in media containing glucose and XylE could still be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 399
    Illegal NgoMIV site found at 571
    Illegal AgeI site found at 922
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None